| Téléchargement | - Voir le manuscrit accepté : Flow through target bacteria capture, lysis and DNA extraction using magnetic clouds (PDF, 474 Kio)
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| Auteur | Rechercher : Malic, Lidija1; Rechercher : Poncelet, Lucas1; Rechercher : Clime, Liviu1; Rechercher : Geissler, Matthias1; Rechercher : Morton, Keith1; Rechercher : Nassif, Christina1; Rechercher : Da Fonte, Dillon1; Rechercher : Veilleux, Gaétan1; Rechercher : Veres, Teodor1 |
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| Affiliation | - Conseil national de recherches du Canada. Dispositifs médicaux
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| Format | Texte, Affiche |
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| Conférence | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Oct. 10-14, 2021, Palm Springs, California [paired with a virtual conference site hosted on-line] |
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| Sujet | magnetic separation; flow-through sample preparation; bacteria capture; nucleic acid extraction |
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| Résumé | A novel sample preparation method is presented which uses suspended magnetic nanoparticle (MNP) assemblies (termed M-cloud) formed within a micropillar array magnetized using an external magnetic field. Composed of aggregated MNPs, the M-cloud serves as a porous capture matrix for target analyte flowing through the array. The concept is applied for in-situ magnetic capture of target bacteria, lysis and DNA extraction performed on a polymer microfluidic chip in a flow-through manner. Quantitative assessment M-cloud performance using river water samples and downstream qPCR revealed that DNA output correlates linearly with the input bacteria concentration, making it possible to confirm E. coli O157:H7 at 10³ cells/mL. |
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| Date de publication | 2022-08 |
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| Maison d’édition | Chemical and Biological Microsystems Society |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-MD-2021-07 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | f7f71094-2647-4369-ac05-4da215c482e3 |
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| Enregistrement créé | 2021-11-18 |
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| Enregistrement modifié | 2022-09-23 |
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