Download | - View accepted manuscript: Flow through target bacteria capture, lysis and DNA extraction using magnetic clouds (PDF, 474 KiB)
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Author | Search for: Malic, Lidija1; Search for: Poncelet, Lucas1; Search for: Clime, Liviu1; Search for: Geissler, Matthias1; Search for: Morton, Keith1; Search for: Nassif, Christina1; Search for: Da Fonte, Dillon1; Search for: Veilleux, Gaétan1; Search for: Veres, Teodor1 |
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Affiliation | - National Research Council of Canada. Medical Devices
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Format | Text, Poster |
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Conference | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Oct. 10-14, 2021, Palm Springs, California [paired with a virtual conference site hosted on-line] |
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Subject | magnetic separation; flow-through sample preparation; bacteria capture; nucleic acid extraction |
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Abstract | A novel sample preparation method is presented which uses suspended magnetic nanoparticle (MNP) assemblies (termed M-cloud) formed within a micropillar array magnetized using an external magnetic field. Composed of aggregated MNPs, the M-cloud serves as a porous capture matrix for target analyte flowing through the array. The concept is applied for in-situ magnetic capture of target bacteria, lysis and DNA extraction performed on a polymer microfluidic chip in a flow-through manner. Quantitative assessment M-cloud performance using river water samples and downstream qPCR revealed that DNA output correlates linearly with the input bacteria concentration, making it possible to confirm E. coli O157:H7 at 10³ cells/mL. |
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Publication date | 2022-08 |
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Publisher | Chemical and Biological Microsystems Society |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NRC-MD-2021-07 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | f7f71094-2647-4369-ac05-4da215c482e3 |
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Record created | 2021-11-18 |
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Record modified | 2022-09-23 |
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