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Flow through target bacteria capture, lysis and DNA extraction using magnetic clouds

From National Research Council Canada

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Affiliation
  1. National Research Council Canada. Medical Devices
FormatText, Poster
Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Oct. 10-14, 2021, Palm Springs, California [paired with a virtual conference site hosted on-line]
Subjectmagnetic separation; flow-through sample preparation; bacteria capture; nucleic acid extraction
Abstract
A novel sample preparation method is presented which uses suspended magnetic nanoparticle (MNP) assemblies (termed M-cloud) formed within a micropillar array magnetized using an external magnetic field. Composed of aggregated MNPs, the M-cloud serves as a porous capture matrix for target analyte flowing through the array. The concept is applied for in-situ magnetic capture of target bacteria, lysis and DNA extraction performed on a polymer microfluidic chip in a flow-through manner. Quantitative assessment M-cloud performance using river water samples and downstream qPCR revealed that DNA output correlates linearly with the input bacteria concentration, making it possible to confirm E. coli O157:H7 at 10³ cells/mL.
Publication date
PublisherChemical and Biological Microsystems Society
In
  • 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), MIA-101.a (August 2022): 213–214.
LanguageEnglish
Peer reviewedYes
NRC numberNRC-MD-2021-07
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Record identifierf7f71094-2647-4369-ac05-4da215c482e3
Record created2021-11-18
Record modified2022-09-23
Date modified: