| DOI | Trouver le DOI : https://doi.org/10.3109/10409239509085140 |
|---|
| Auteur | Rechercher : Jackson, Michael1; Rechercher : Mantsch, Henry H.1 |
|---|
| Affiliation | - Conseil national de recherches Canada. Institut du biodiagnostic du CNRC
|
|---|
| Format | Texte, Article |
|---|
| Sujet | infrared spectroscopy; proteins; secondary structure; quantitation; IT |
|---|
| Résumé | Fourier transform infrartd (FTIR) spectroscopy is an established tool for the structural characterization of proteins. However, many potential pitfalls exist for the unwary investigator. In this review we critically assess the application of FIlR spectroscopy to the determination of protein structure by (1) outlining the principles underlying protein secondary structure determination by FZZR spectroscopy. (2) highhghting the situations in which FZZR spectroscopy should be considered the technique of choice, (3) discussing the manner in which experiments should be conducted to derive as much physiologically relevant information as possible, and (4) outlining current methods for the determination of secondary structure from infrared spectm of proteins. |
|---|
| Date de publication | 1995 |
|---|
| Dans | |
|---|
| Langue | anglais |
|---|
| Publications évaluées par des pairs | Oui |
|---|
| Numéro du CNRC | NRC-IBD-173 |
|---|
| Numéro NPARC | 9148110 |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | ede2bf1b-4314-45b2-b7ea-28eef2a9e98e |
|---|
| Enregistrement créé | 2009-06-25 |
|---|
| Enregistrement modifié | 2020-04-29 |
|---|