| DOI | Resolve DOI: https://doi.org/10.3109/10409239509085140 |
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| Author | Search for: Jackson, Michael1; Search for: Mantsch, Henry H.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
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| Format | Text, Article |
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| Subject | infrared spectroscopy; proteins; secondary structure; quantitation; IT |
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| Abstract | Fourier transform infrartd (FTIR) spectroscopy is an established tool for the structural characterization of proteins. However, many potential pitfalls exist for the unwary investigator. In this review we critically assess the application of FIlR spectroscopy to the determination of protein structure by (1) outlining the principles underlying protein secondary structure determination by FZZR spectroscopy. (2) highhghting the situations in which FZZR spectroscopy should be considered the technique of choice, (3) discussing the manner in which experiments should be conducted to derive as much physiologically relevant information as possible, and (4) outlining current methods for the determination of secondary structure from infrared spectm of proteins. |
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| Publication date | 1995 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-IBD-173 |
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| NPARC number | 9148110 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ede2bf1b-4314-45b2-b7ea-28eef2a9e98e |
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| Record created | 2009-06-25 |
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| Record modified | 2020-04-29 |
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