DOI | Trouver le DOI : https://doi.org/10.1016/0005-2736(79)90123-8 |
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Auteur | Rechercher : Casal, Hector L.1; Rechercher : Smith, Ian C. P.1; Rechercher : Cameron, David G.1; Rechercher : Mantsch, Henry H.1 |
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Affiliation | - Conseil national de recherches du Canada
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Format | Texte, Article |
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Sujet | Lipid reorganization; Fourier transform; Phase transition; (Infrared difference) |
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Résumé | The first application of infrared difference spectroscopy to the study of a natural biological membrane is described. Perdeuterated palmitic acid was incorporated biosynthetically into the lipids of the plasma membrane of Acholeplasma laidlawii and the temperature-induced structural rearrangement of the endogenous lipids monitored via their C2H vibrational modes. Changes in infrared parameters were studied between 0 and 50°C and contrasted with those occurring in the model membrane system of 1,2-diperdeuteropalmitoyl-sn-glycero-3-phosphocholine. The phase transition of the biomembrane occurs over a 20°C range with the temperature of the maximum rate of change of absorbance coinciding with that of the sharp phase transition of the model membrane. |
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Date de publication | 1979-01-05 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro du CNRC | NRCC 16933 |
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Numéro NPARC | 23001278 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | cc74a748-9dcf-462c-94a4-753b91443ca5 |
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Enregistrement créé | 2017-01-13 |
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Enregistrement modifié | 2020-03-13 |
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