| DOI | Resolve DOI: https://doi.org/10.1016/0005-2736(79)90123-8 |
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| Author | Search for: Casal, Hector L.1; Search for: Smith, Ian C. P.1; Search for: Cameron, David G.1; Search for: Mantsch, Henry H.1 |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | Lipid reorganization; Fourier transform; Phase transition; (Infrared difference) |
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| Abstract | The first application of infrared difference spectroscopy to the study of a natural biological membrane is described. Perdeuterated palmitic acid was incorporated biosynthetically into the lipids of the plasma membrane of Acholeplasma laidlawii and the temperature-induced structural rearrangement of the endogenous lipids monitored via their C2H vibrational modes. Changes in infrared parameters were studied between 0 and 50°C and contrasted with those occurring in the model membrane system of 1,2-diperdeuteropalmitoyl-sn-glycero-3-phosphocholine. The phase transition of the biomembrane occurs over a 20°C range with the temperature of the maximum rate of change of absorbance coinciding with that of the sharp phase transition of the model membrane. |
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| Publication date | 1979-01-05 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 16933 |
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| NPARC number | 23001278 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | cc74a748-9dcf-462c-94a4-753b91443ca5 |
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| Record created | 2017-01-13 |
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| Record modified | 2020-03-13 |
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