| DOI | Trouver le DOI : https://doi.org/10.1039/C6AY00407E |
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| Auteur | Rechercher : Aviram, Lilach Yishai1; Rechercher : McCooeye, Margaret1; Rechercher : Mester, Zoltan1Identifiant ORCID : https://orcid.org/0000-0002-2377-2615 |
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| Affiliation | - Conseil national de recherches Canada. Science des mesures et étalons
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| Format | Texte, Article |
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| Résumé | In this paper we describe a rapid method for microscale microwave assisted acid hydrolysis followed by quantitative amino acid analysis, using liquid chromatography mass spectrometry UPLC-ESI-MS (QTOF) without derivatization. Such an approach allows substantial decrease in the amount of yeast sample (less than 1 mg) required for effective analysis. The vapor phase hydrolysis approach was compared to condensed phase hydrolysis and all the microwave parameters were optimized for 16 amino acids. Separation was achieved on a BEH C-18 column. Limits of detection and quantitation (LODs/LOQs), using standard solutions, were in the sub-ppb range. The method was successfully applied to the determination and quantitation of methionine and selenomethionine by isotope dilution and standard addition methods in yeast on a yeast certified reference material sample. |
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| Date de publication | 2016-05-04 |
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| Maison d’édition | Royal Society of Chemistry |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23000365 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c337c563-9ee2-4f32-a5bd-a1df661582aa |
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| Enregistrement créé | 2016-07-11 |
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| Enregistrement modifié | 2020-03-16 |
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