| DOI | Resolve DOI: https://doi.org/10.1039/C6AY00407E |
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| Author | Search for: Aviram, Lilach Yishai1; Search for: McCooeye, Margaret1; Search for: Mester, Zoltan1ORCID identifier: https://orcid.org/0000-0002-2377-2615 |
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| Affiliation | - National Research Council of Canada. Measurement Science and Standards
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| Format | Text, Article |
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| Abstract | In this paper we describe a rapid method for microscale microwave assisted acid hydrolysis followed by quantitative amino acid analysis, using liquid chromatography mass spectrometry UPLC-ESI-MS (QTOF) without derivatization. Such an approach allows substantial decrease in the amount of yeast sample (less than 1 mg) required for effective analysis. The vapor phase hydrolysis approach was compared to condensed phase hydrolysis and all the microwave parameters were optimized for 16 amino acids. Separation was achieved on a BEH C-18 column. Limits of detection and quantitation (LODs/LOQs), using standard solutions, were in the sub-ppb range. The method was successfully applied to the determination and quantitation of methionine and selenomethionine by isotope dilution and standard addition methods in yeast on a yeast certified reference material sample. |
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| Publication date | 2016-05-04 |
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| Publisher | Royal Society of Chemistry |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23000365 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c337c563-9ee2-4f32-a5bd-a1df661582aa |
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| Record created | 2016-07-11 |
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| Record modified | 2020-03-16 |
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