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A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF02534359
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada
FormatTexte, Article
Sujetalpha tocopherol; lipid; animal cell; blood and hemopoietic system; erythrocyte; human cell; liver; plasma; rat; cholesterol; erythrocyte membrane; fatty acids; in vitro; lipids; liver; male; rats, inbred strains; support, non-U. S. Gov't; vitamin E
Résumé
A new, general method for lipid extraction and measurement of vitamin E/total lipid ratios in tissue and cell samples has been developed. The new extraction procedure use a combination of sodium dodecylsulfate, ethanol and n-heptane, and is mild, clean, convenient, efficient and rapid (≤5 min). The efficiency of the new method has been confirmed for human plasma, red blood cells and rat liver homogenate by the comparison of the yields of vitamin E, O-acyl lipid and cholesterol with the yields obtained following conventional extraction procedures. Extraction efficiency also has been confirmed for multilamenllar vesicles composed of known quantities of vitamin E, egg lecithin and cholesterol. © 1985 American Oil Chemists' Society (AOCS).
Date de publication
Dans
  • Lipids 20, nº 1 (1985) : 29–39.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21276567
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Identificateur de l’enregistrementbd05b02a-fbe1-43bd-aad4-82307f07f990
Enregistrement créé2015-10-13
Enregistrement modifié2020-03-17
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