A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

From National Research Council Canada

DOIResolve DOI: http://doi.org/10.1007/BF02534359
AuthorSearch for: 1 ; Search for: 1 ; Search for: 1 
Name affiliation
  1. National Research Council Canada
TypeArticle
Journal titleLipids
ISSN0024-4201
Volume20
Issue1
Pages29–39; # of pages: 11
Subjectalpha tocopherol; lipid; animal cell; blood and hemopoietic system; erythrocyte; human; human cell; liver; plasma; rat; Animal; Cholesterol; Erythrocyte Membrane; Fatty Acids; Human; In Vitro; Lipids; Liver; Male; Methods; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Vitamin E
Abstract
A new, general method for lipid extraction and measurement of vitamin E/total lipid ratios in tissue and cell samples has been developed. The new extraction procedure use a combination of sodium dodecylsulfate, ethanol and n-heptane, and is mild, clean, convenient, efficient and rapid (≤5 min). The efficiency of the new method has been confirmed for human plasma, red blood cells and rat liver homogenate by the comparison of the yields of vitamin E, O-acyl lipid and cholesterol with the yields obtained following conventional extraction procedures. Extraction efficiency also has been confirmed for multilamenllar vesicles composed of known quantities of vitamin E, egg lecithin and cholesterol. © 1985 American Oil Chemists' Society (AOCS).
Publication date
LanguageEnglish
Peer reviewedYes
NPARC number21276567
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