A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

DOI	Resolve DOI: https://doi.org/10.1007/BF02534359
Author	Search for: Burton, G.W.¹; Search for: Webb, A.¹; Search for: Ingold, K. U.¹
Name affiliation	National Research Council of Canada
Format	Text, Article
Subject	alpha tocopherol; lipid; animal cell; blood and hemopoietic system; erythrocyte; human cell; liver; plasma; rat; cholesterol; erythrocyte membrane; fatty acids; in vitro; lipids; liver; male; rats, inbred strains; support, non-U. S. Gov't; vitamin E
Abstract	A new, general method for lipid extraction and measurement of vitamin E/total lipid ratios in tissue and cell samples has been developed. The new extraction procedure use a combination of sodium dodecylsulfate, ethanol and n-heptane, and is mild, clean, convenient, efficient and rapid (≤5 min). The efficiency of the new method has been confirmed for human plasma, red blood cells and rat liver homogenate by the comparison of the yields of vitamin E, O-acyl lipid and cholesterol with the yields obtained following conventional extraction procedures. Extraction efficiency also has been confirmed for multilamenllar vesicles composed of known quantities of vitamin E, egg lecithin and cholesterol. © 1985 American Oil Chemists' Society (AOCS).
Publication date	1985
In	Lipids 20, no. 1 (1985): 29–39.
Language	English
Peer reviewed	Yes
NPARC number	21276567
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Record identifier	bd05b02a-fbe1-43bd-aad4-82307f07f990
Record created	2015-10-13
Record modified	2020-03-17