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Solid-phase extraction (SPE) and liquid chromatography-mas spectrometry (LC-MS) for the determination of free fatty acids in shellfish

AuteurRechercher : ; Rechercher : ; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches Canada. Science des mesures et étalons
FormatTexte, Article
Sujetfree fatty acids; shellfish; lipid extraction; solid phase extraction; liquid chromatography–mass spectrometry
Résumé
A novel analytical protocol for the determination of free fatty acids (FFAs; saturated, monounsaturated and polyunsaturated) in shellfish using electrospray ionisation and liquid chromatography–mass spectrometry (LC–MS) is described. Total lipids were extracted from four commercially important shellfish species using chloroform-methanol in a modification of the traditionally used Bligh and Dyer method. FFAs were recovered from lipidic shellfish extracts by solid-phase extraction (SPE) on an aminopropyl-silica column using a 98:2 v/v diethyl ether (DEE)-acetic acid solution. Ether extracts containing the FFAs were evaporated and reconstituted in 70:30 v/v methanol–chloroform before analysis by LC–MS. The limits of quantification (LOQs) of the method ranged from 60 to 560 ?g g?1 wet weight depending on the different FFAs determined with selected ion monitoring (SIM). Results demonstrate that LC–MS is well suited for identification and quantification of FFAs in shellfish and negates the use of sample derivatisation required in gas chromatographic analysis.
Date de publication
Conditions d’utilisation
  • Crown Copyright © 2007 Published by Elsevier B.V. All rights reserved.
Dans
  • Journal of Chromatography A 1145, nº 1-2 (23 mars 2007) : 51–57.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42614
1251
Numéro NPARC3538322
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrementb48ffdfe-32c4-4172-90b5-d7766a334552
Enregistrement créé2009-03-01
Enregistrement modifié2020-05-10

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