Dominant inhibition of awn development by a putative zinc‐finger transcriptional repressor expressed at the B1 locus in wheat

Par Conseil national de recherches du Canada

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DOI	Trouver le DOI : https://doi.org/10.1111/nph.16154
Auteur	Rechercher : Huang, Daiqing¹; Rechercher : Zheng, Qian¹; Rechercher : Melchkart, Tancey¹; Rechercher : Bekkaoui, Yasmina¹; Rechercher : Konkin, David J. F.¹; Rechercher : Kagale, Sateesh¹; Rechercher : Martucci, Martial; Rechercher : You, Frank M.; Rechercher : Clarke, Martha; Rechercher : Adamski, Nikolai M.; Rechercher : Chinoy, Catherine; Rechercher : Steed, Andrew; Rechercher : Mccartney, Curt A.; Rechercher : Cutler, Adrian J.¹; Rechercher : Nicholson, Paul; Rechercher : Feurtado, J. Allan¹
Affiliation	Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
Format	Texte, Article
Sujet	awn; awnletted; B1 Locus; C2H2; EAR motif; transcription repression; wheat; zinc finger
Résumé	Awns, bristle‐like structures extending from grass lemmas, provide protection against predators, contribute to photosynthesis and aid in grain dispersal. In wheat, selection of awns with minimal extension, termed awnletted, has occurred during domestication by way of loci that dominantly inhibit awn development, such as Tipped1 (B1), Tipped2 (B2), and Hooded (Hd). Here we identify and characterize the B1 gene. B1 was identified using bulked segregant RNA‐sequencing of an F2 durum wheat population and through deletion mapping of awned bread wheat mutants. Functional characterization was accomplished by gene overexpression while haplotype analyses assessed B1 polymorphisms and genetic variation. Located on chromosome 5A, B1 is a C2H2 zinc finger encoding gene with ethylene‐responsive element binding factor‐associated amphiphilic repression (EAR) motifs. Constitutive overexpression of B1 in awned wheat produced an awnletted phenotype with pleiotropic effects on plant height and fertility. Transcriptome analysis of B1 overexpression plants suggests a role as transcriptional repressor, putatively targeting pathways involved in cell proliferation. Haplotype analysis revealed a conserved B1 coding region with proximal polymorphisms and supported the contention that B1 is mainly responsible for awnletted wheats globally. B1, predominantly responsible for awn inhibition in wheat, encodes a C2H2 zinc finger protein with EAR motifs which putatively functions as a transcriptional repressor.
Date de publication	2019-08-30
Maison d’édition	New Phytologist Trust Wiley
Licence	Creative Commons, Attribution 4.0 Générique (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/deed.fr
Dans	New Phytologist 225, nº 1 : 340–355.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRC-ACRD-PDB017
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	b0964a8b-998f-45bc-b1fc-699dc1aa758b
Enregistrement créé	2019-11-29
Enregistrement modifié	2020-09-22

Date de modification :: 2024-04-18