Dominant inhibition of awn development by a putative zinc‐finger transcriptional repressor expressed at the B1 locus in wheat

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DOI	Resolve DOI: https://doi.org/10.1111/nph.16154
Author	Search for: Huang, Daiqing¹; Search for: Zheng, Qian¹; Search for: Melchkart, Tancey¹; Search for: Bekkaoui, Yasmina¹; Search for: Konkin, David J. F.¹; Search for: Kagale, Sateesh¹; Search for: Martucci, Martial; Search for: You, Frank M.; Search for: Clarke, Martha; Search for: Adamski, Nikolai M.; Search for: Chinoy, Catherine; Search for: Steed, Andrew; Search for: Mccartney, Curt A.; Search for: Cutler, Adrian J.¹; Search for: Nicholson, Paul; Search for: Feurtado, J. Allan¹
Affiliation	National Research Council of Canada. Aquatic and Crop Resource Development
Format	Text, Article
Subject	awn; awnletted; B1 Locus; C2H2; EAR motif; transcription repression; wheat; zinc finger
Abstract	Awns, bristle‐like structures extending from grass lemmas, provide protection against predators, contribute to photosynthesis and aid in grain dispersal. In wheat, selection of awns with minimal extension, termed awnletted, has occurred during domestication by way of loci that dominantly inhibit awn development, such as Tipped1 (B1), Tipped2 (B2), and Hooded (Hd). Here we identify and characterize the B1 gene. B1 was identified using bulked segregant RNA‐sequencing of an F2 durum wheat population and through deletion mapping of awned bread wheat mutants. Functional characterization was accomplished by gene overexpression while haplotype analyses assessed B1 polymorphisms and genetic variation. Located on chromosome 5A, B1 is a C2H2 zinc finger encoding gene with ethylene‐responsive element binding factor‐associated amphiphilic repression (EAR) motifs. Constitutive overexpression of B1 in awned wheat produced an awnletted phenotype with pleiotropic effects on plant height and fertility. Transcriptome analysis of B1 overexpression plants suggests a role as transcriptional repressor, putatively targeting pathways involved in cell proliferation. Haplotype analysis revealed a conserved B1 coding region with proximal polymorphisms and supported the contention that B1 is mainly responsible for awnletted wheats globally. B1, predominantly responsible for awn inhibition in wheat, encodes a C2H2 zinc finger protein with EAR motifs which putatively functions as a transcriptional repressor.
Publication date	2019-08-30
Publisher	New Phytologist Trust Wiley
Licence	Creative Commons, Attribution 4.0 Generic (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
In	New Phytologist 225, no. 1: 340–355.
Language	English
Peer reviewed	Yes
NRC number	NRC-ACRD-PDB017
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Record identifier	b0964a8b-998f-45bc-b1fc-699dc1aa758b
Record created	2019-11-29
Record modified	2020-09-22

Date modified:: 2024-07-21