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| DOI | Trouver le DOI : https://doi.org/10.1016/j.jlr.2024.100668 |
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| Auteur | Rechercher : Li, Jianjun1; Rechercher : Stupak, Jacek1; Rechercher : Haqqani, Arsalan S.1; Rechercher : Harris, Greg1; Rechercher : Zhou, Hongyan1; Rechercher : Williamson, Sam1; Rechercher : Chen, Rui1Identifiant ORCID : https://orcid.org/0000-0003-3149-9492; Rechercher : Xu, H. Howard; Rechercher : Chen, Wangxue1Identifiant ORCID : https://orcid.org/0000-0003-0958-7728 |
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| Affiliation | - Conseil national de recherches Canada. Thérapeutique en santé humaine
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| Bailleur de fonds | Rechercher : National Research Council Canada |
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| Format | Texte, Article |
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| Sujet | FAIMS; lipidomics; mass spectrometry; phospholipid; NPLC; Acinetobacter baumannii; bacterial infection |
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| Résumé | The recent advances in mass spectrometry (MS) technologies have enabled comprehensive lipid profiling in biological samples. However, the robustness and efficiency of MS-based lipidomics is compromised by the complexity of biological samples. High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a technology that can continuously transmit one type of ion, independent of mass-to-charge ratio. Here we present the development and application of LC-FAIMS-MS/MS based platform for untargeted lipidomics. We used 3 optimally balanced compensation voltages, i.e., 29 V, 34 V and 39 V, to analyse all subclasses of glycerophospholipids. The reproducibility of the method was evaluated using reference standards. The reproducibility of retention times ranged from 0.9 to 1.5 % RSD; whereas RSD values of 5-10 % were observed for peak areas. More importantly, the coupling of a FAIMS device can significantly improve the robustness and efficiency. We exploited this NPLC-FAIMS-HRMS to analyze the serum lipid profiles in mice infected intranasally with Acinetobacter baumannii. The temporal profiles of serum lipids after A. baumannii inoculation were obtained for 4 h, 8 h and 24 h. We found that nearly all ether PC and ether PE lipids were significantly decreased 8 h after inoculation. The resultant volcano plot illustrated the distribution of 28 increased and 28 decreased lipid species in mouse sera 24 h after inoculation. We also found that a single ether PE composition can comprise multiple isomeric structures, and the relative abundance of each isomer could be quantified using the newly developed NPLC-FAIMS-PRM method. |
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| Date de publication | 2024-10-10 |
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| Maison d’édition | Elsevier |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | S0022227524001731 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 6e810b7b-e8a5-439a-81bd-e1e253f6abd6 |
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| Enregistrement créé | 2024-10-24 |
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| Enregistrement modifié | 2025-11-06 |
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