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| DOI | Resolve DOI: https://doi.org/10.1016/j.jlr.2024.100668 |
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| Author | Search for: Li, Jianjun1; Search for: Stupak, Jacek1; Search for: Haqqani, Arsalan S.1; Search for: Harris, Greg1; Search for: Zhou, Hongyan1; Search for: Williamson, Sam1; Search for: Chen, Rui1ORCID identifier: https://orcid.org/0000-0003-3149-9492; Search for: Xu, H. Howard; Search for: Chen, Wangxue1ORCID identifier: https://orcid.org/0000-0003-0958-7728 |
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| Affiliation | - National Research Council Canada. Human Health Therapeutics
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| Funder | Search for: National Research Council Canada |
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| Format | Text, Article |
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| Subject | FAIMS; lipidomics; mass spectrometry; phospholipid; NPLC; Acinetobacter baumannii; bacterial infection |
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| Abstract | The recent advances in mass spectrometry (MS) technologies have enabled comprehensive lipid profiling in biological samples. However, the robustness and efficiency of MS-based lipidomics is compromised by the complexity of biological samples. High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a technology that can continuously transmit one type of ion, independent of mass-to-charge ratio. Here we present the development and application of LC-FAIMS-MS/MS based platform for untargeted lipidomics. We used 3 optimally balanced compensation voltages, i.e., 29 V, 34 V and 39 V, to analyse all subclasses of glycerophospholipids. The reproducibility of the method was evaluated using reference standards. The reproducibility of retention times ranged from 0.9 to 1.5 % RSD; whereas RSD values of 5-10 % were observed for peak areas. More importantly, the coupling of a FAIMS device can significantly improve the robustness and efficiency. We exploited this NPLC-FAIMS-HRMS to analyze the serum lipid profiles in mice infected intranasally with Acinetobacter baumannii. The temporal profiles of serum lipids after A. baumannii inoculation were obtained for 4 h, 8 h and 24 h. We found that nearly all ether PC and ether PE lipids were significantly decreased 8 h after inoculation. The resultant volcano plot illustrated the distribution of 28 increased and 28 decreased lipid species in mouse sera 24 h after inoculation. We also found that a single ether PE composition can comprise multiple isomeric structures, and the relative abundance of each isomer could be quantified using the newly developed NPLC-FAIMS-PRM method. |
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| Publication date | 2024-10-10 |
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| Publisher | Elsevier |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0022227524001731 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 6e810b7b-e8a5-439a-81bd-e1e253f6abd6 |
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| Record created | 2024-10-24 |
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| Record modified | 2025-11-06 |
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