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In vitro transformation of paralytic shellfish toxins in the clams Mya arenaria and Protothaca staminea

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.hal.2005.05.005
AuteurRechercher : 1; Rechercher : 2; Rechercher : 3
Affiliation
  1. Conseil national de recherches du Canada. Initiative en génomique et en santé du CNRC
  2. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
  3. Conseil national de recherches du Canada. Programme d'aide à la recherche industrielle
FormatTexte, Article
SujetCarbamoylase; clamsc Mya arenaria; paralytic shellfish toxins; Protothaca staminea
Résumé
Dissected tissues of two clam species, the Pacific littleneck, Protothaca staminea, and soft-shell, Mya arenaria, were evaluated for in vitro conversion of paralytic shellfish poisoning (PSP) toxins. Tissue homogenates were incubated with purified PSP toxins to determine the time-course of toxin conversion. The effects of boiling and addition of a natural reductant (glutathione) on toxin conversion were also assessed. For P. staminea, the digestive gland showed the greatest capacity for biotransformation, followed by gill, but mantle, adductor muscle, and siphon tissues exhibited very low conversion. In this species, the production of decarbamoyl derivatives was much greater from low potency N-sulfocarbamoyl toxins than from carbamate analogues. Decarbamolyation exhibited apparent specificity for ?-epimers of all toxin substrates and this reaction was inhibited by boiling. Glutathione-mediated desulfation was tissue specific and had apparent specificity for ?-epimers. These observations on P. staminea suggest that the above reactions are enzyme-mediated. In contrast, there was little toxin conversion in M. arenaria homogenates, but even this low activity was heat-labile and thus likely enzyme-mediated.
Date de publication
Dans
  • Harmful Algae 5, nº 1 (janvier 2006) : 79–90.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 55548
1511
Numéro NPARC3538137
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Identificateur de l’enregistrement4375b784-c498-4c54-b0bb-2fd9e050e168
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-22
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