| DOI | Resolve DOI: https://doi.org/10.1002/rcm.2650 |
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| Author | Search for: Sleno, Lekha1; Search for: Daneshfar, Rambod1; Search for: Eckert, Gunter P.; Search for: Muller, Walter E.; Search for: Volmer, Dietrich A.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | methods; mass spectrometry; Hypericum perforatum; St. John's wort |
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| Abstract | Active phloroglucinol constituents of Hypericum perforatum (St. John's wort) extracts, hyperforin and adhyperforin, have been studied following ion activation using tandem mass spectrometry (MS/MS) and complemented by accurate mass measurements. These two compounds were readily analyzed as protonated and deprotonated molecules with electrospray ionization. MS/MS and MS3 data from a quadrupole-linear ion trap tandem mass spectrometer were employed to elucidate fragmentation pathways. Fourier transform ion cyclotron resonance measurements afforded excellent mass accuracies for the confirmation of elemental formulae of product ions formed via infrared multiphoton dissociation and sustained off-resonance irradiation collision-induced dissociation. Fragmentation schemes have been devised for the dissociation of hyperforin and adhyperforin in negative and positive ion modes. This information is expected to be especially valuable for the characterization of related compounds, such as degradation products, metabolites and novel synthetic analogs of hyperforin. |
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| Publication date | 2006-09-30 |
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| Terms of use | - Copyright © 2006 John Wiley & Sons, Ltd.
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | NRC-ACRD-55906 |
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| NRC number | 1621 |
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| NPARC number | 3538410 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c36773eb-543d-4b8e-9fb7-96381d98ed5e |
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| Record created | 2009-03-01 |
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| Record modified | 2020-04-22 |
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