Identification of a transcript encoding a soluble form of toll-like receptor 5 (TLR5) in Atlantic salmon during Aeromonas salmonicida infection

From National Research Council Canada

Alternative title	Identification of a transcript encoding a soluble form of toll-like receptor 5 (TLR5) in the liver of Atalantic salmon infected with Aeromonas salmonicida
DOI	Resolve DOI: https://doi.org/10.1016/j.vetimm.2005.05.013
Author	Search for: Tsoi, Stephen¹; Search for: Park, Kyoung C.¹; Search for: Kay, Helen H.; Search for: O'Brien, Timothy J.; Search for: Eszter, Podor; Search for: Sun, Genlou; Search for: Douglas, Susan E.¹; Search for: Brown, Laura L.¹; Search for: Johnson, Stewart C.¹
Affiliation	National Research Council of Canada. NRC Institute for Marine Biosciences
Format	Text, Article
Subject	soluble toll-like receptor; leucine-rich-repeats; LRR; inflammation; Atlantic salmon
Abstract	Toll-like receptors (TLRs) are involved in the innate immune response against microbial pathogens in vertebrates and insects. The extracellular region of a TLR recognizes pathogen-associated molecules, while the intracellular region initiates the signaling pathway leading to immune response. Membrane-bound TLRs have been found in most vertebrates, but few soluble forms have been reported. A novel transcript corresponding to a portion of a soluble TLR was identified in liver of infected Atlantic salmon. The complete coding sequence of this TLR was obtained and BLASTN analysis showed the highest sequence identity to a recently described full-length cDNA sequence of a soluble TLR5 from rainbow trout (GenBank Accession No.: AB062504). The deduced protein is 40% identical to the mammalian counterpart of the leucine-rich repeat (LRR)/LRR-like motifs of TLR5. Based on the structure of human TLRs, it contains 21 LRRs with conserved LxxLxLxxNxxxxxxxFxxL pattern. Since TLR5 is essential for the recognition of bacterial flagellins, we hypothesize that flagellin and perhaps some other pathogen-derived factors from Aeromonas salmonicida bind to this soluble TLR through an unknown binding domain within the LRR.
Publication date	2006-01-01
Terms of use	Copyright © 2005 Elsevier B.V. All rights reserved.
In	Veterinary Immunology and Immunopathology 109, no. 1-2 (1 January 2006): 183–187.
Language	English
Peer reviewed	Yes
NRC number	NRCC 55506 1641
NPARC number	3538328
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Record identifier	ac471292-ff11-41c9-b867-e8310357ac41
Record created	2009-03-01
Record modified	2020-04-22

Date modified:: 2024-12-05