| Download | - View final version: Phaseic acid: an endogenous and reversible inhibitor of glutamate receptors in mouse brain (PDF, 8 MB)
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| DOI | Resolve DOI: https://doi.org/10.1074/jbc.M116.756429 |
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| Author | Search for: Hou, Sheng T.1; Search for: Jiang, Susan X.1; Search for: Zaharia, L. Irina2; Search for: Han, Xiumei2; Search for: Benson, Chantel L.2; Search for: Slinn, Jacqueline1; Search for: Abrams, Suzanne R.2 |
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| Name affiliation | - National Research Council of Canada. Human Health Therapeutics
- National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | phaseic acid; abscisic acid; UPLC/MS/MS; endothelial cells; choroid plexus; cerebrospinal fluid; cerebral ischemia |
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| Abstract | Phaseic acid (PA) is a phytohormone regulating important physiological functions in higher plants. Here, we show the presence of naturally occurring (-)-PA in mouse and rat brains. (-)-PA is exclusively present in the choroid plexus and the cerebral vascular endothelial cells. Purified (-)-PA has no toxicity and protects cultured cortical neurons against glutamate toxicity through reversible inhibition of glutamate receptors. Focal occlusion of the middle cerebral artery (MCAO) elicited a significant induction in (-)-PA expression in the CSF, but not in the peripheral blood. Importantly, (-)-PA induction only occurred in the penumbra area, indicting a protective role of PA in the brain. Indeed, elevating (-)-PA level in the brain reduced ischemic brain injury, while reducing (-)-PA level using a monoclonal antibody against (-)-PA increased ischemic injury. Collectively, these studies showed for the first time that (-)-PA is an endogenous neuroprotective molecule capable of reversible inhibiting glutamate receptors during ischemic brain injury. |
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| Publication date | 2016-11-18 |
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| Publisher | American Society for Biochemistry and Molecular Biology |
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| Licence | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 23001033 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 3d585ba4-1b44-4f27-9795-6bb4efa62f4e |
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| Record created | 2016-12-01 |
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| Record modified | 2021-04-26 |
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