| DOI | Resolve DOI: https://doi.org/10.1073/pnas.94.4.1453 |
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| Author | Search for: Lau, Peter C. K.1; Search for: Wang, Ying1; Search for: Patel, Ashok1; Search for: Labbé, Diane1; Search for: Bergeron, Hélène1; Search for: Brousseau, Roland1; Search for: Konishi, Yasuo1; Search for: Rawlings, Merriann |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | environmental; two-component regulatory system; signal transduction; aromatic hydrocarbons; Pseudomonas |
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| Abstract | The two-component signal transduction pathways in bacteria use a histidine–aspartate phosphorelay circuit to mediate cellular changes in response to environmental stimuli. Here we describe a novel two-component todST system, which activates expression of the toluene degradation (tod) pathway in Pseudomonas putida F1. The todS gene is predicted to encode a sensory hybrid kinase with two unique properties—a basic region leucine zipper dimerization motif at the N terminus and a duplicated histidine kinase motif. Evidence from a synthetic peptide model suggests that TodS binds as a dimer to a pseudopalindromic sequence (5'-TGACTCA), which resembles the recognition sequence of the eukaryotic transcription factors Fos and Jun. These results provide additional evidence that bacteria and eukaryotes share common regulatory motifs. The todT gene product, a response regulator, was overproduced as a fusion protein in Escherichia coli, and the purified protein was found to bind specifically to a 6-bp palindromic DNA structure in the tod control region. The phosphorylated form of TodT appears to be the activator of tod structural genes. This is the first report of a two-component system that regulates aromatic metabolism in bacteria. |
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| Publication date | 1997-02-18 |
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| In | |
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| Note | UI - 97188489 |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | 40468 |
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| NPARC number | 3538647 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 2bcb70a5-fb97-47a1-864e-ea6c502fbe7a |
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| Record created | 2009-03-01 |
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| Record modified | 2021-05-10 |
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