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A bacterial basic region leucine zipper histidine kinase regulating toluene degradation

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1073/pnas.94.4.1453
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher :
Affiliation
  1. Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
FormatTexte, Article
Sujetenvironmental; two-component regulatory system; signal transduction; aromatic hydrocarbons; Pseudomonas
Résumé
The two-component signal transduction pathways in bacteria use a histidine–aspartate phosphorelay circuit to mediate cellular changes in response to environmental stimuli. Here we describe a novel two-component todST system, which activates expression of the toluene degradation (tod) pathway in Pseudomonas putida F1. The todS gene is predicted to encode a sensory hybrid kinase with two unique properties—a basic region leucine zipper dimerization motif at the N terminus and a duplicated histidine kinase motif. Evidence from a synthetic peptide model suggests that TodS binds as a dimer to a pseudopalindromic sequence (5'-TGACTCA), which resembles the recognition sequence of the eukaryotic transcription factors Fos and Jun. These results provide additional evidence that bacteria and eukaryotes share common regulatory motifs. The todT gene product, a response regulator, was overproduced as a fusion protein in Escherichia coli, and the purified protein was found to bind specifically to a 6-bp palindromic DNA structure in the tod control region. The phosphorylated form of TodT appears to be the activator of tod structural genes. This is the first report of a two-component system that regulates aromatic metabolism in bacteria.
Date de publication
Dans
  • Proceedings of the National Academy of Sciences of the United States of America (PNAS) 94, nº 4 (18 février 1997) : 1453–1458.
NoteUI - 97188489
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRC40468
Numéro NPARC3538647
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistrement2bcb70a5-fb97-47a1-864e-ea6c502fbe7a
Enregistrement créé2009-03-01
Enregistrement modifié2021-05-10
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