National Research Council of Canada. Aquatic and Crop Resource Development
aglycone; daunorubicin; digitoxosyl phenanthroviridin; gyrase inhibitor; lysine; unclassified drug; absorption spectroscopy; amino acid metabolism; cancer cell culture; cytotoxicity; fermentation; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; high performance liquid chromatography; human cell; liquid chromatography; mass spectrometry; nuclear Overhauser effect; protein structure; proton nuclear magnetic resonance; Streptomyces venezuelae; tandem mass spectrometry
The jadomycin-derived compound l-digitoxosyl-phenanthroviridin was isolated from fermentations of Streptomyces venezuelae ISP5230 grown in nutrient-deficient media with l-lysine as the sole nitrogen source. Structural elucidation was accomplished using a combination of high-resolution MS, LC-MS/MS, and 1D- and 2D-NMR. The compound was evaluated against the National Cancer Institute (NCI) 60 human tumor cell line screen in both the one-dose and five-dose screens, and cytotoxicity was compared to a small library of jadomycin analogues to probe the structure-activity relationship.
Journal of Natural Products78, no. 8 (23 July 2015): 1942–1948.