| DOI | Resolve DOI: https://doi.org/10.1016/j.cbpb.2013.02.007 |
|---|
| Author | Search for: Chimsung, N.1; Search for: Lall, S. P.1; Search for: Tantikitti, C.; Search for: Verlhac-Trichet, V.; Search for: Milley, J. E.1 |
|---|
| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
|
|---|
| Format | Text, Article |
|---|
| Subject | high density lipoprotein cholesterol; low density lipoprotein cholesterol; very low density lipoprotein; absorption; blood sampling; density gradient centrifugation; dietary intake; fish; polyacrylamide gel electrophoresis; separation technique; sucrose density gradient centrifugation; cholesterol, dietary; cholesterol, VLDL; lipoproteins; lipoproteins, HDL; Xanthophylls |
|---|
| Abstract | The effect of dietary cholesterol on astaxanthin (Ax) absorption and transport in the plasma of Atlantic salmon was investigated. Under controlled conditions, three experimental diets, non-pigmented diet (NPD), NPD with 40mg Axkg-1, and NPD with 40mg Axkg-1 and 2% cholesterol, were fed to juvenile salmon reared in sea water. After 12weeks, blood was collected and plasma separated for analysis of plasma Ax and cholesterol content. In addition, plasma samples from each group of fish were fractionated into lipoproteins using a sucrose density gradient and ultracentrifugation. The apolipoprotein components of VLDL, LDL and HDL from each sample fraction were separated using SDS-PAGE. The addition of 2% cholesterol to the Ax-containing diet significantly increased the concentration of Ax and cholesterol in fish plasma. The protein-rich fraction was found to be the major carrier of Ax in salmon plasma. Cholesterol supplementation significantly increased Ax in plasma and VLDL as well as increasing plasma cholesterol. The VLDL fraction showed the most significant change in fish fed diet supplemented with cholesterol resulting in higher levels of Ax in this lipoprotein. The results clearly show that dietary cholesterol had a significant effect on the Ax transport process in the blood. |
|---|
| Publication date | 2013-03-01 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NPARC number | 21270396 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 02c1e43f-ec53-44e5-a143-f5289b14d3e2 |
|---|
| Record created | 2014-02-06 |
|---|
| Record modified | 2020-04-22 |
|---|
