| Téléchargement | - Voir la version finale : Photouncaging of ceramides promotes reorganization of liquid-ordered domains in supported lipid bilayers (PDF, 1.1 Mio)
- Voir le manuscrit accepté : Photouncaging of ceramides promotes reorganization of liquid-ordered domains in supported lipid bilayers (PDF, 5.3 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1021/la3039158 |
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| Auteur | Rechercher : Carter Ramirez, Daniel M.1; Rechercher : Pitre, Spencer P.2; Rechercher : Kim, Young Ah; Rechercher : Bittman, Robert; Rechercher : Johnston, Linda J.1 |
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| Affiliation | - Conseil national de recherches du Canada. Science des mesures et étalons
- Conseil national de recherches du Canada. Institut Steacie des sciences moléculaires du CNRC
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| Format | Texte, Article |
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| Résumé | 6-Bromo-7-hydroxycoumarin (Bhc)-caged ceramide (Cer) analogs were incorporated into supported lipid bilayers containing a mixture of coexisting liquid-ordered (L₀) and liquid-disordered (Ld) phases. The release of N-palmitoyl and N-butanoyl-d-erythro-sphingosine (C16- and C4-Cer) by the photolysis of caged Cers using long-wavelength UV light was studied using a combination of atomic force microscopy and fluorescence microscopy. This approach demonstrated the ability to generate Cer with spatial and temporal control, providing an alternative method to the enzymatic generation of Cer. The generation of C16-Cer from Bhc-C16-Cer disrupted the L₀ domains, with the incorporation of small fluid-phase regions and the disappearance of some smaller domains. Cer-rich gel-phase domains were not observed, in contrast to results reported by either direct Cer incorporation or enzymatic Cer generation. The photorelease of C4-Cer from Bhc-C4-Cer resulted in qualitatively similar changes in bilayer morphology, with the disappearance of some L₀ domains and no evidence of Cer-rich gel domains but with a smaller height difference between the ordered and disordered phases. |
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| Date de publication | 2013-02-12 |
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| Maison d’édition | ACS Publications |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23000586 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | efe4c16f-42cb-483f-9423-b9dd955d6a32 |
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| Enregistrement créé | 2016-08-03 |
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| Enregistrement modifié | 2020-09-09 |
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