| Auteur | Rechercher : John, U.1; Rechercher : Quilliam, M. A.1; Rechercher : Medlin, L.; Rechercher : Cembella, A. D.1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
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| Format | Texte, Article |
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| Conférence | The Ninth International Conference on Harmful Algal Blooms, Feb. 7-11, 2000, Hobart, Australia |
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| Résumé | The effects of physiological status on spirolide production were studied in nutrient-replete batch cultures of a toxic strain of the dinoflagellate Alexandrium ostenfeldii. Although complete ce11 synchronisation was not achieved by dark adaptation, the concentration of motile vegetative cells apparently increased in the light anddecreased in the dark. The concentration of extracted chlorophyll a followed the same trend as the ce11 concentration, with no apparent shift in the amount of chlorophyll a per ce11 in relation to the light/dark (L/D) phase. Analysis of spirolides by liquid chromatography coupled with mass spectrometry (LC-MS) showed that the toxin profile did not vary significantly over the L/D cycle, and consisted primarily of a des-methyl-C derivative (>90% molar), with minor constituents C, C3, D, D3 and des-methyl-D. The total spirolide concentration per unit culture volume was directly related to the concentration of cells and chlorophyll a, but there was a dramatic increase in ce11 quota of spirolides at the beginning of the dark phase and a corresponding decrease in the light. The biosynthesis of these polyketide-derived metabolites is apparently govemed by light-dependent events during the ce11 division cycle. |
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| Date de publication | 2001 |
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| Maison d’édition | International Oceanographic Commission (UNESCO) |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23001117 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | e8f08d49-b40e-4f43-9130-6bef5834939b |
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| Enregistrement créé | 2016-12-13 |
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| Enregistrement modifié | 2020-03-27 |
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