Pressure-induced exclusion of a local anesthetic from model and nerve membranes

DOI	Trouver le DOI : https://doi.org/10.1021/bi00400a003
Auteur	Rechercher : Auger, Michèle¹; Rechercher : Jarrell, Harold C.¹; Rechercher : Smith, Ian C. P.¹; Rechercher : Wong, Patrick T. T.¹; Rechercher : Siminovitch, David (Dave)¹; Rechercher : Mantsch, Henry H.¹
Affiliation	Conseil national de recherches Canada
Format	Texte, Article
Résumé	Because it is well established that the anesthetic state can be reversed by pressure, a number of molecular theories that have been proposed for the mechanism of action of both local and general anesthetics can be tested by varying the pressure. Using Fourier transform infrared spectroscopy, we report here the first direct observation of the expulsion from lipid bilayers of a local anesthetic, tetracaine, by pressure. Moreover, we establish for the first time that this phenomenon is common to both model membranes and to myelinated and unmyelinated nerve membranes, vindicating the utility of model membrane systems. A distinctive feature of this behavior in model systems is that, in saturated phosphatidylcholines at high pH, expulsion only occurs in the presence of cholesterol, whose ordering effect on the acyl chains evidently assists pressure in squeezing the anesthetic out of the bilayer. This pressure-induced phenomenon may provide insight into the molecular mechanisms underlying the antagonistic effect of pressure against anesthesia.
Date de publication	1987-12
Dans	Biochemistry 26, nº 26 : 8513–8516.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro du CNRC	NRCC 28296 AUGER1987
Numéro NPARC	9381215
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	e427a7d2-9e9a-472a-a927-ebbec683b93f
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-03-17

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Date de modification :: 2026-03-09