| DOI | Trouver le DOI : https://doi.org/10.1016/S0022-0248(01)01155-1 |
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| Auteur | Rechercher : Sivaraman, J.1; Rechercher : Li, Yunge1; Rechercher : Plamondon, Josée1; Rechercher : Larocque, Robert1; Rechercher : Raymond, Stéphane1; Rechercher : Sauvé, Véronique1; Rechercher : Smith, Christopher1; Rechercher : Boju, Lorena1; Rechercher : Schrag, Joseph1; Rechercher : Matte, Allan1; Rechercher : Gaasterland, Terry; Rechercher : Cygler, Miroslaw1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | genome; pharmaceutical; A1. Biocrystallization; A1. Crystal structure; A1. Purification; A1. X-ray diffraction |
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| Résumé | A pilot project based on gene targets selected from the genome of E. coli has been initiated with 38 genes for initial cloning. Of these, 18 proteins have been purified to date and some crystals were obtained for twelve of them. Of these, four proteins yielded crystals diffracting to a sufficiently high resolution to warrant structural investigation. We have determined 3-D structures of three of these proteins using Se-Met labeling and MAD methods, while the structure of the fourth one was simultaneously determined by another group. To manage the parallel work on many proteins by several researchers it became necessary to create a searchable database containing the pertinent information about every stage of the work. |
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| Date de publication | 2001-08-15 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | 44826 |
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| Numéro NPARC | 3539584 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | d82392fd-859b-41c8-9c67-eb715fc5862f |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-03-27 |
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