| Téléchargement | - Voir le manuscrit accepté : Multilabeling biomolecules at a single site. 1. Synthesis and characterization of a dendritic label for electrochemiluminescence assays (PDF, 808 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1021/ac034664d |
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| Auteur | Rechercher : Zhou, Ming1; Rechercher : Roovers, Jacques2; Rechercher : Robertson, Gilles P.2; Rechercher : Grover, Chander P.1 |
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| Affiliation | - Conseil national de recherches Canada. Institut des étalons nationaux de mesure du CNRC
- Conseil national de recherches Canada. Institut de technologie des procédés chimiques et de l'environnement du CNRC
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| Format | Texte, Article |
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| Résumé | Ultrasensitive bioanalytical assays are of great value for early detection of human diseases and pathogens. The sensitivities of immunoassays and DNA probing can be enhanced by multilabeling the biorecognition partner used for affinity-based assays. However, the bioreactivity of biomolecules is affected by a high degree of multilabeling at multiple functional sites. It is proposed that dendritic scaffoldings be used to link multiple signal-generating units to a single site with potentially minimum impact on the bioaffinity. A prototype label, a zeroth-generation dendron, bearing three [Ru(bpy)₃]²⁺ units for electrochemiluminescence (ECL) assays was synthesized and characterized preliminarily by spectroscopic, electrochemical, and ECL methods. No evidence of interaction between the neighboring [Ru(bpy)₃]²⁺ units in the label molecule was found from these characterizations. Both the photoluminescence and ECL of the prototype label have features very similar to those of mononuclear [Ru- (bpy)₃]²⁺ compounds. Labeling a model protein with a triad of [Ru(bpy)₃]²⁺ at one NH₂ position was demonstrated. The results reported here provide support to applying the proposed multilabeling strategy to affinitybased bioanalytical assays. |
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| Date de publication | 2003-12 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 51678 |
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| Numéro NPARC | 14542472 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | d795fddf-c5ee-4f0a-a89f-f0f12ff122f8 |
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| Enregistrement créé | 2010-03-01 |
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| Enregistrement modifié | 2020-04-02 |
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