Purine and pyrimidine biosynthesis in methanogenic bacteria

DOI	Trouver le DOI : https://doi.org/10.1007/BF00307767
Auteur	Rechercher : Choquet, C.¹; Rechercher : Richards, J.¹; Rechercher : Patel, G.¹; Rechercher : Sprott, G.¹
Affiliation	Conseil national de recherches Canada. Institut des sciences biologiques du CNRC
Format	Texte, Article
Résumé	Following long-term labeling with [1-13C]acetate, [2-13C]acetate, 13CO2, H13COOH, or 13CH3OH, NMR spectroscopy was used to determine the labeling patterns of the purified ribonucleosides of Methanospirillum hungatei, Methanococcus voltae, Methanobrevibacter smithii, Methanosphaera stadtmanae, Methanosarcina barkeri and Methanobacterium bryantii. Major differences were observed among the methanogens studied, specifically at carbon positions 2 and 8 of the purines, positions at which one-carbon carriers are involved during synthesis. In Methanospirillum hungatei and Methanosarcina barkeri, the labcl at both positions came from carbon atom C-2 of acetate, as predicted from known eubacterial pathways, whereas in Methanococcus voltae and Methanobacterium bryantii both originated from CO2. In Methanosphaera stadtmanae grown in the presence of formate, the C-2 of purines originated exclusively from formate and the C-8 was labeled by the C-2 of acetate. When grown in media devoid of formate, the C-2 of the purine ring originated mainly from the C-2 of acetate and in part from CH3OH. In Methanobrevibacter smithii grown in the presence of formate, C-2 and C-8 of purines were derived from CO2 and/or formate. The labeling patterns obtained for pyrimidines are consistent with the biosynthetic pathways common to eubacteria and eucaryotes.
Date de publication	1994
Dans	Archives of Microbiology 161, nº 6 (1994) : 471–480.
Langue	anglais
Numéro du CNRC	CHOQUET1994C NRCC 37383
Numéro NPARC	9378739
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	d6e30f49-1a92-4bac-9ea9-7cd0cbd29461
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-04-27

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Date de modification :: 2026-08-15