A regenerating ultrasensitive electrochemical impedance immunosensor for the detection of adenovirus

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1016/j.bios.2014.12.032
Auteur	Rechercher : Lin, Donghai; Rechercher : Tang, Thompson; Rechercher : Harrison, D. Jed¹; Rechercher : Lee, William E.; Rechercher : Jemere, Abebaw B.¹
Affiliation	Conseil national de recherches du Canada. Institut national de nanotechnologie
Format	Texte, Article
Sujet	Cyclic voltammetry; Desorption; Electrochemical electrodes; Fiber optic sensors; Gold; Metal nanoparticles; Nanoparticles; Self assembled monolayers; Spectroscopy; Viruses; X ray photoelectron spectroscopy; 11-mercaptoundecanoic acid; Adenovirus; Covalent immobilization; Electrochemical impedance; Linear dynamic ranges; Modified electrodes; Reductive desorption; Self-assembled layers; Electrochemical impedance spectroscopy; gold nanoparticle; undecanoic acid; virus antibody; chemical modification; chemical reaction; concentration (parameters); cyclic potentiometry; electrochemical impedance spectroscopy; electrode; immobilization; immunosensor; limit of detection; virus detection; Human adenovirus type 5
Résumé	We report on the development of a regenerable sensitive immunosensor based on electrochemical impedance spectroscopy for the detection of type 5 adenovirus. The multi-layered immunosensor fabrication involved successive modification steps on gold electrodes: (i) modification with self-assembled layer of 1,6-hexanedithiol to which gold nanoparticles were attached via the distal thiol groups, (ii) formation of self-assembled monolayer of 11-mercaptoundecanoic acid onto the gold nanoparticles, (iii) covalent immobilization of monoclonal anti-adenovirus 5 antibody, with EDC/NHS coupling reaction on the nanoparticles, completing the immunosensor. The immunosensor displayed a very good detection limit of 30 virus particles/ml and a wide linear dynamic range of 105. An electrochemical reductive desorption technique was employed to completely desorb the components of the immunosensor surface, then re-assemble the sensing layer and reuse the sensor. On a single electrode, the multi-layered immunosensor could be assembled and disassembled at least 30 times with 87% of the original signal intact. The changes of electrode behavior after each assembly and desorption processes were investigated by cyclic voltammetry, electrochemical impedance spectroscopy and X-ray photoelectron spectroscopy techniques.
Date de publication	2014-12-16
Dans	Biosensors and Bioelectronics 68 (16 décembre 2014) : 129–134.
Langue	anglais
Publications évaluées par des pairs	Oui
Numéro NPARC	21275836
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	d4a99124-a009-4e86-b524-02b93676adde
Enregistrement créé	2015-07-14
Enregistrement modifié	2020-04-22

Date de modification :: 2024-11-09