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Viability, cell division and microcallus formation of barley microspores in culture

DOITrouver le DOI : https://doi.org/10.1007/BF00234694
AuteurRechercher : 1
Affiliation
  1. Conseil national de recherches Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetglucose; sugar; sucrose; glycol; cell division
Résumé
Barley microspores were viable when cultured in a sugarless medium. Adding 2g of glucose to 1l of this medium resulted in a significant reduction in the frequency of viable microspores. The frequency of viable microspores was further reduced when 50g of cellobiose, glucose, maltose, melezitose, raffinose or sucrose were added to 1l of the culture medium containing 2g/l glucose. Adding 50g of melibiose, Ficoll, polyethylene glycol (PEG) or a combination 50g each of Ficoll and PEG to 1I of the medium containing 2g/l glucose had very little effect on the viability of the microspores. Up to 66% of the viable microspores were able to divide and many of these developed into microcalli in the basal medium complemented with melibiose, maltose, melezitose, raffinose, Ficoll, PEG or a combination of Ficoll with PEG. Sucrose, cellobiose and glucose added in large quantities inhibited cell division in microspores or destabilized the microspores and only very few of them developed into microcalli. The microcalli in the PEG, Ficoll, Ficoll-PEG and melibiose media were smaller in size than those grown in the melezitose, maltose and raffinose media. Sustained cell division and microcallus formation were observed in a medium with melibiose or maltose as sole source of sugars.
Date de publication
Maison d’éditionSpringer Nature
Dans
  • Plant Cell Reports 12-12, nº 7-8 (mai 1993).
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF00234694
NRCC-35126
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrementc5c9e8f9-a7a8-4688-8f07-da17d6f45edb
Enregistrement créé2023-11-06
Enregistrement modifié2023-11-06

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