| DOI | Trouver le DOI : https://doi.org/10.1016/0304-4211(74)90097-2 |
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| Auteur | Rechercher : Kartha, K. K.1; Rechercher : Michayluk, M. R.1; Rechercher : Kao, K. N.1; Rechercher : Gamborg, O. L.1; Rechercher : Constabel, F.1 |
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| Affiliation | - Conseil national de recherches du Canada. Laboratoire régional des prairies
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| Format | Texte, Article |
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| Résumé | Protoplasts from mesophyll cells of rape plants (Brassica napus L. cv. Zephyr) were isolated by enzymatic removal of cell walls in an osmoticum consisting of sorbitol and mannitol. A two-step enzyme treatment involving 0.5% each of Onozuka P1500 cellulase and Rhozyme HP150 hemicellulase followed by 0.5% each of Driselase and hemicellulase at pH 6.2 resulted in the production of viable protoplasts in 80–90% yield. The protoplasts were cultured in droplets of B5 medium in petri dishes at a light intensity of 100 lux and 26°. They were able to regenerate cell walls. Cell division was apparent after 30 h of culturing and by 60 h up to 38% of the protoplasts had divided once. Cell clusters were formed within 15 days of culturing. The calli originating from cell clusters were induced to differentiate and to form complete plants. |
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| Date de publication | 1974-10 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Identificateur | 0304421174900972 |
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| Numéro du CNRC | NRCC-14034 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c470f91e-aa3d-488f-b0ab-1a2954126b01 |
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| Enregistrement créé | 2023-07-06 |
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| Enregistrement modifié | 2023-07-11 |
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