| DOI | Trouver le DOI : https://doi.org/10.1016/j.jbiotec.2016.05.014 |
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| Auteur | Rechercher : Kryachko, Y.1; Rechercher : Semler, D.; Rechercher : Vogrinetz, J.; Rechercher : Lemke, M.; Rechercher : Links, M. G.1; Rechercher : McCarthy, E. L.1; Rechercher : Haug, B.1; Rechercher : Hemmingsen, S. M.1 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Sujet | interfacial tension; microbially enhanced oil recovery (MEOR); microbial community; cpn60 |
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| Résumé | Microorganisms indigenous to an oil reservoir were grown in media containing either sucrose or proteins in four steel vessels under anoxic conditions at 30 °C and 8.3 MPa for 30 days, to enrich biosurfactant producers. Fermentation of substrate was possible in the protein-containing medium and either fermentation or respiration through reduction of sulfate occurred in the sucrose-containing medium. Growth of microorganisms led to 3.4–5.4-fold surface tension reduction indicating production of biosurfactants in amounts sufficient for enhancement of gas-driven oil recovery. Analysis of sequenced cpn60 amplicons showed that Pseudomonas sp. highly similar to biosurfactant producing P. fluorescens and to Pseudomonas sp. strain TKP predominated, and a bacterium highly similar to biosurfactant producing Bacillus mojavensis was present in vessels. Analysis of 16S rDNA amplicons allowed only genus-level identification of these bacteria. Thus, cpn60-amplicon analysis was a more relevant tool for identification of putative biosurfactant producers than 16S rDNA-amplicon analysis. |
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| Date de publication | 2016-05-19 |
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| Maison d’édition | Elsevier |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23001951 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c4499902-694c-4c25-99b2-374e4a2944c6 |
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| Enregistrement créé | 2017-06-27 |
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| Enregistrement modifié | 2020-03-16 |
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