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Determination of vanadium in biological fluids using HR-ICP-MS

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DOITrouver le DOI : https://doi.org/10.1039/b207563f
AuteurRechercher : 1Identifiant ORCID : https://orcid.org/0000-0002-6896-8603; Rechercher : 1; Rechercher : ; Rechercher :
Affiliation
  1. Conseil national de recherches Canada. Institut des étalons nationaux de mesure du CNRC
FormatTexte, Article
Résumé
A simple, fast and sensitive method is described for the determination of vanadium in biological fluids using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). Samples were diluted 20-fold in 0.3% HNO3. Spectral interference from 35Cl16O+, present in urine or serum matrices, was completely resolved from the vanadium major isotope of 51V+ at medium resolution of 4000. Quantitation of V in urine and serum was achieved by the method of additions using standards prepared in a pooled urine or serum sample. Mass offset and lock mass (40Ar16O+) options were employed for more accurate and precise results. Better than 2.0% RSD was obtained with a 200 pg ml−1 V spike added to a 20-fold diluted urine or serum matrix, reflecting one day stability. Vanadium concentrations in the range of <10 to 1500 pg ml−1 and <10 to 760 pg ml−1 were determined in the urine and serum samples, respectively. Good day-to-day precision of less than 4.0% RSD was typical for both sample matrices. Spike recoveries of 99 ± 2 and 98 ± 3% (one standard deviation, n = 3) were obtained in the urine and serum, respectively. An in-house CRM (SLRS-4: river water) was analyzed daily, yielding a V concentration of 0.337 ± 0.010 ng ml−1 (one standard deviation, n = 35), in good agreement with the certified value of 0.32 ± 0.03 ng ml−1. A detection limit (3 s) of 10 pg ml−1 was estimated for V in reconstituted urine and serum samples.
Date de publication
Dans
  • Journal of Analytical Atomic Spectrometry 17, nº 10 (2002) : 1300–1303.
Langueanglais
Numéro du CNRCNRC-INMS-120
Numéro NPARC8899905
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Identificateur de l’enregistrementc3dbbb89-a5c0-4ac2-9cb3-5d30383da7de
Enregistrement créé2009-04-22
Enregistrement modifié2024-08-12

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