| DOI | Trouver le DOI : https://doi.org/10.1002/elps.200406046 |
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| Auteur | Rechercher : Luong, John H. T.1; Rechercher : Male, Keith B.1; Rechercher : Mazza, Alberto1; Rechercher : Masson, Luke1; Rechercher : Brousseau, Roland1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | environmental; proteins; Escherichia coli |
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| Résumé | Bacillus thuringiensis and recombinant Escherichia coli proteinaceous protoxins were subject to proteolysis and analyzed by capillary electrokinetic chromatography. Three resulting toxins (65 kDa) were baseline-resolved within 22 min using a 10 mM borate, pH 11 separation buffer consisting of 25 mM sodium dodecyl sulfate (SDS) and 30 mm phytic acid. The toxins displayed differential interactions with the SDS and phytic acid phases to effect their separation. The ion-pairing interaction between the analyte and phytic acid was also useful in preventing adsorption to the capillary walls and thus enhanced separation resolution and efficiency. The use of electrokinetic chromatography allows achievement of the separation in a significantly shorter time than conventional high-performance liquid chromatography (HPLC) using a diethylaminoethyl (DEAE) weak-anion exchanger. |
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| Date de publication | 2004 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-BRI-47192 |
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| Numéro NPARC | 3538718 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | c105fd18-d4b0-4cf7-a15d-74a184964616 |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2022-11-18 |
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