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Selection and identification of single domain antibody fragments from camel heavy-chain antibodies

Par Conseil national de recherches du Canada

AuteurRechercher : ; Rechercher : ; Rechercher : ; Rechercher : ; Rechercher :
FormatTexte, Article
Sujetamino acid sequence; antibody; antibody affinity; antibody specificity; antigen; bacteriophages; binding; binding sites; binding sites, antibody; camels; chain; cloning, molecular; domain; epitope mapping; gene library; genetics; identification; immunoglobulin heavy chains; immunology; light; metabolism; molecular sequence data; polymerase chain reaction; protein; recombinant proteins
Résumé
Functional heavy-chain gamma-immunoglobulins lacking light chains occur naturally in Camelidae. We now show the feasibility of immunising a dromedary, cloning the repertoire of the variable domains of its heavy-chain antibodies and panning, leading to the successful identification of minimum sized antigen binders. The recombinant binders are expressed well in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens. This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins
Date de publication
Dans
  • FEBS Lett. 414, nº 3 (15 septembre 1997) : 521–526.
Langueanglais
Numéro du CNRCARBABI1997
Numéro NPARC9363882
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Identificateur de l’enregistrementb60221b6-7c81-427a-90b8-e7edf5717069
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-20
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