| Téléchargement | - Voir le manuscrit accepté : The Bt gene cry2Aa2 driven by a tissue specific ST-LS1 promoter from potato effectively controls Heliothis virescens (PDF, 667 Kio)
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| DOI | Trouver le DOI : https://doi.org/10.1007/s11248-004-7714-3 |
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| Auteur | Rechercher : Zaidi, Mohsin Abbas; Rechercher : Mohammadi, Mojtaba; Rechercher : Postel, Sandra; Rechercher : Masson, Luke1; Rechercher : Altosaar, Illimar |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | environmental; protein; plants; mortality; proteins |
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| Résumé | Expression of the Cry2Aa2 protein was targeted specifically to the green tissues of transgenic tobacco Nicotiana tabacum cv. Xanthi plants. This deployment was achieved by using the promoter region of the gene encoding the Solanum tuberosum leaf and stem specific (ST-LS1) protein. The accumulated levels of toxin in the leaves were found to be effective in achieving 100 mortality of Heliothis virescens larvae. The levels of Cry2Aa2 expression in the leaves of these transgenic plants were up to 0.21 of the total soluble proteins. Bioassays with R1 transgenic plants indicated the inheritance of cry2Aa2 in the progeny plants. Tissue-specific expression of the Bt toxin in transgenic plants may help in controlling the potential occurrence of insect resistance by limiting the amount of toxin to only predated tissues. The results reported here validate the use of the ST-LS1 gene promoter for a targeted expression of Bt toxins in green tissues of plants. |
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| Date de publication | 2005 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | 47215 |
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| Numéro NPARC | 3539874 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b5030c7a-572e-4075-8bf9-7b7a9121118b |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2023-04-24 |
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