| Téléchargement | - Voir la version finale : An automated screening platform for improving the responsiveness of genetically encoded Ca²⁺ biosensors in mammalian cells (PDF, 2.9 Mio)
- Voir les données supplémentaires : An automated screening platform for improving the responsiveness of genetically encoded Ca²⁺ biosensors in mammalian cells (PDF, 347 Kio)
|
|---|
| DOI | Trouver le DOI : https://doi.org/10.1039/D4SD00138A |
|---|
| Auteur | Rechercher : Zhao, Yufeng1Identifiant ORCID : https://orcid.org/0000-0002-1788-7969; Rechercher : Shen, YiIdentifiant ORCID : https://orcid.org/0000-0003-4117-0279; Rechercher : Veres, Teodor1Identifiant ORCID : https://orcid.org/0000-0002-9594-8042; Rechercher : Campbell, Robert E. |
|---|
| Affiliation | - Conseil national de recherches Canada. Dispositifs médicaux
|
|---|
| Bailleur de fonds | Rechercher : Natural Sciences and Engineering Research Council of Canada; Rechercher : Japan Society for the Promotion of Science; Rechercher : Canadian Institutes of Health Research |
|---|
| Format | Texte, Article |
|---|
| Résumé | Genetically-encoded, fluorescent protein (FP)-based biosensors are powerful tools for imaging dynamic cellular activities. Directed evolution is a highly effective method for developing enhanced versions of FP-based biosensors, but the screening process is laborious and time-consuming. Mammalian cell-based screening with electrical stimulation methods has been successful in accurately selecting variants of biosensors for imaging neuronal activities. We introduce an automated mammalian cell screening platform utilizing a fluorescence microscope and a liquid dispenser to enable the screening of biosensor responsiveness to chemical stimulation. We demonstrated the effectiveness of this platform in improving the response of a red fluorescent biosensor for Ca²⁺, K-GECO, for detection of histamine-induced changes in Ca²⁺ concentration. This method should be applicable to any FP-based biosensor that responds to pharmacological treatment or other exogenous chemical stimulation, simplifying efforts to develop biosensors tailored for specific applications in diverse biological contexts. |
|---|
| Date de publication | 2024-07-18 |
|---|
| Maison d’édition | Royal Society of Chemistry |
|---|
| Licence | |
|---|
| Dans | |
|---|
| Langue | anglais |
|---|
| Publications évaluées par des pairs | Oui |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | b3cfd84f-4c34-49a3-b0ab-72ec9f76cdf9 |
|---|
| Enregistrement créé | 2025-06-12 |
|---|
| Enregistrement modifié | 2025-06-12 |
|---|
