| DOI | Trouver le DOI : https://doi.org/10.1557/jmr.2012.323 |
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| Auteur | Rechercher : Parisien, A.; Rechercher : Al-Zarka, F.; Rechercher : Hussack, G.1; Rechercher : Baranova, E.A.; Rechercher : Thibault, J.; Rechercher : Qingdao Lan, C. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | Adsorption cycle; Average diameter; Binding capacities; Ferromagnetic behaviors; magnetic; Magnetic adsorbents; Magnetic characterization; Magnetization-saturation; Modified polyol method; Nickel nanoparticles; Nucleating agents; Palladium chloride; Polyvinyl pyrrolidone; Protective agents; Protein purification; Single domains; Adsorbents; Adsorption; Alcohols; Amino acids; Antibodies; Chlorine compounds; Ethylene glycol; Magnetic domains; Nanomagnetics; Nanoparticles; Nickel; Nickel compounds; Palladium; Purification; Recombinant proteins; Saturation magnetization; X ray diffraction; Biosynthesis |
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| Résumé | Nickel nanoparticles (NNPs) synthesized by a modified polyol method using ethylene glycol as a reducing agent, palladium chloride as a nucleating agent, and polyvinylpyrrolidone (PVP) as a protective agent were investigated as a potential magnetic adsorbent for the purification of hexahistidine-tagged (His6-tagged) recombinant proteins. The synthesis resulted in nanoparticles having an average diameter of 6828 nm. The x-ray diffraction pattern confirmed the presence of nickel metal, as well as the presence of unreacted nickel (II) hydroxide Ni(OH)2. Magnetic characterization showed that a magnetization saturation of 39.3 electromagnetic unit (emu)/g at 20,000 Oersted (Oe) was reached rapidly and that the material exhibited ferromagnetic behavior. Protein purification results showed that the synthesized NNPs were highly selective for binding to a His6-tagged recombinant protein single-domain antibody ToxA5.1. In addition, NNPs were used for four adsorption cycles without significant binding capacity losses. These particles have shown great potential such as being easily synthesized, cost-effective, and highly selective magnetic adsorbents for the purification of His6-tagged recombinant proteins. © Copyright © Materials Research Society 2012. |
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| Date de publication | 2012 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269398 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | b1c3d0e3-befa-4853-86c1-742479160b0e |
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| Enregistrement créé | 2013-12-12 |
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| Enregistrement modifié | 2020-04-21 |
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