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| DOI | Trouver le DOI : https://doi.org/10.1002/pei3.10044 |
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| Auteur | Rechercher : Li, Qiang1Identifiant ORCID : https://orcid.org/0000-0003-0705-2850; Rechercher : Shen, Wenyun1; Rechercher : Mavraganis, Ioannis1; Rechercher : Wang, Liping1; Rechercher : Gao, Peng1Identifiant ORCID : https://orcid.org/0000-0002-6586-4307; Rechercher : Gao, Jie; Rechercher : Cram, Dustin1; Rechercher : Li, Yifeng2; Rechercher : Liu, Ziying2; Rechercher : Fowler, David Brian; Rechercher : Pan, Youlian2Identifiant ORCID : https://orcid.org/0000-0002-0158-0081; Rechercher : Zou, Jitao1 |
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| Affiliation | - Conseil national de recherches Canada. Développement des cultures et des ressources aquatiques
- Conseil national de recherches Canada. Technologies numériques
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| Format | Texte, Article |
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| Sujet | wheat; phosphatidylglycerol; trans‐16:1; cold acclimation |
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| Résumé | In plant cells, phosphatidylglycerol (PG) in the chloroplast has a characteristic trans-∆3-hexadecenoic acid (t16:1) at the sn-2 position. The t16:1 content in wheat leaf tissues decreases during cold treatment, but the significance of this fatty acid compositional change and the underlying biochemical mechanism remains poorly understood. Using a large collection of wheat cultivars displaying a varying capacity of freezing tolerance, we show for the first time under field conditions that this low temperature induced t16:1 change is associated with winter hardiness. To explore the metabolic mechanism responsible for the reduction of t16:1, we performed detailed lipid analysis and comparative transcriptome study with four selected wheat lines under cold acclimation. Our results show that wheat leaf tissues experience a gradual decrease in chloroplast lipid pathway activity during cold acclimation and that the decline in chloroplast lipid synthesis manifests itself in the decrease of t16:1 in PG. Comparative RNA-seq analyses with leaf tissues further reveal concerted transcriptome shifts indicating a rebalancing of chloroplast and cytosolic lipid synthesis during cold acclimation. Our study, thus, provides mechanistic understanding on chloroplast lipid adjustments as a “molecular ideotype” and the t16:1 change as a specific metabolite marker for screening freezing tolerance in wheat. |
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| Date de publication | 2021-05-17 |
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| Maison d’édition | Wiley New Phytologist Foundation |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-ACRD-PDB0121 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | a92c9547-bf45-4911-a384-b9cc758bd6b0 |
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| Enregistrement créé | 2021-05-18 |
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| Enregistrement modifié | 2022-08-15 |
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