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Gouvernement du Canada / Government of Canada

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Histological assessment of angiogenesis in the hypoxic central nervous system

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/978-1-4939-0320-7_14
AuteurRechercher : ; Rechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Thérapeutique en santé humaine
FormatTexte, Article
Sujetangiogenesis; animal cell; animal tissue; antibody labeling; brain tissue; central nervous system; confocal laser scanning microscope; female; fluorescence; hypoxia; image analysis; immunofluorescence; in vivo study; mouse; quantitative analysis; tissue section; Brain; Cell Hypoxia; Cerebrovascular Circulation; Cryoultramicrotomy; Fluorescent Antibody Technique, Indirect; Mice; Microscopy, Fluorescence; Neovascularization, Physiologic
Résumé
Angiogenesis, the sprouting of new capillaries from preexisting vessels, is an integral part of both normal development and numerous pathological conditions such as tumor growth, inflammation, and stroke. The development of angiogenesis assays has been critical in understanding this process in both the context of disease and normal physiology. With the growing availability of antibodies against angiogenic markers as well as advances in microscopy and imaging analysis software, a more comprehensive assessment of the angiogenesis process is beginning to take form (Milner et al., Stroke 39:191-197, 2008; Freitas-Andrade et al., J Cereb Blood Flow Metab 32:663-675, 2012; Li et al., Glia 58:1157-1167, 2010; Dore-Duffy and LaManna, Antioxid Redox Signal 9:1363-1371, 2007). This chapter describes an in vivo method of inducing brain angiogenesis in mice by chronic exposure to mild hypoxia. In addition, a detailed procedure of quantifying angiogenesis using multiple immunofluorescent labeling of mouse brain tissue sections is also presented.
Date de publication
Dans
  • Methods in Molecular Biology 1135 (2014) : 157–175.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC21272806
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Identificateur de l’enregistrementa66537f2-aa73-4d56-ace9-b76e2e0aa181
Enregistrement créé2014-12-03
Enregistrement modifié2020-04-22
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