Adding polyvinylpyrrolidone to low level protein samples significantly improves peptide recovery in FASP digests: an inexpensive and simple modification to the FASP protocol

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DOI	Trouver le DOI : https://doi.org/10.1016/j.jprot.2020.104000
Auteur	Rechercher : Tremblay, Tammy-Lynn¹; Rechercher : Hill, Jennifer J.¹
Affiliation	Conseil national de recherches Canada. Thérapeutique en santé humaine
Bailleur de fonds	Rechercher : National Research Council Canada. Human Health Therapeutics Research Centre
Format	Texte, Article
Description physique	5 p.
Sujet	filter-aided sample preparation; polyvinylpyrrolidone; proteomics; sample preparation
Résumé	Filter-aided sample preparation (FASP) remains a popular choice for proteomic sample preparation, particularly for its ability to produce a ‘clean’ peptide sample clear of large molecule contaminants. However, sample loss continues to be a problem particularly for sample inputs that contain less than ten micrograms of protein. Here, we describe that the simple addition of a polymer, polyvinylpyrrolidone-40 (PVP-40) to the protein sample prior to FASP digest significantly improves peptide recovery and identifications, especially with lower level sample inputs. PVP-FASP produces clean samples which required no additional sample clean-up prior to nanoLC-MS analysis. In addition, PVP-FASP is compatible with other FASP modifications, including the use of sodium deoxycholate (DOC) to improve trypsin digestion.
Date de publication	2020-10-02
Maison d’édition	Elsevier
Licence	Attribution - Pas d'Utilisation Commerciale - Pas de Modification 4.0 International (CC BY-NC-ND 4.0) https://creativecommons.org/licenses/by-nc-nd/4.0/deed.fr
Dans	Journal of Proteomics 230, nº C, 104000 (6 janvier 2021).
Langue	anglais
Publications évaluées par des pairs	Oui
Identificateur	S1874391920303687
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	9db0930d-2b17-4e68-89c9-46454856dc5a
Enregistrement créé	2022-09-02
Enregistrement modifié	2025-12-18

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Date de modification :: 2026-03-16