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Preparation of large bivalve specimens for scanning electron microscopy using hexamethyldisilazane (HMDS)

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.2983/0730-8000(2006)25[639:POLBSF]2.0.CO;2
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Sujetbivalve; scanning electron microscopy; method; hexamethyldisilazane (HMDS); ciliation; shrinkage
Résumé
Treatment with hexamethyldisilazane (HMDS) for drying specimens for Scanning Electron Microscopy (SEM) is described for relatively large (>20 mm shell height, SH) individuals of two scallop species, Placopecten magellanicus and Argopecten irradians. The traditionally used method of critical point drying (CPD) has been applied successfully for smaller specimens (~350 μm up to 15 mm SH); however, occasional rupture of the mantle occurred in these specimens. Furthermore, size limitation of the CPD chamber did not permit preparation of larger individuals. An alternative experimental protocol using HMDS was tested, which successfully preserves the morphology of the bivalve feeding organs, mantle surface and the cilia of the two scallop species with negligible shrinkage and few artifacts. Another benefit of this method is that the number of specimens and sample size is not restricted.
Date de publication
Dans
  • Journal of Shellfish Research 25, nº 2 (1 août 2006) : 639–641.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42613
1465
Numéro NPARC3538379
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Identificateur de l’enregistrement96dc868b-9bdf-40a6-82c7-e9abfd56b91e
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-22
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