Téléchargement | - Voir la version finale : mPUMA: a computational approach to microbiota analysis by de novo assembly of operational taxonomic units based on protein-coding barcode sequences (PDF, 746 Kio)
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DOI | Trouver le DOI : https://doi.org/10.1186/2049-2618-1-23 |
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Auteur | Rechercher : Links, Matthew G; Rechercher : Chaban, Bonnie; Rechercher : Hemmingsen, Sean M.1; Rechercher : Muirhead, Kevin1; Rechercher : Hill, Janet E. |
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Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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Format | Texte, Article |
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Sujet | Operational taxonomic unit; Automated sequence analysis pipeline; 60 kDa chaperonin; Cpn 60; Microbial profiling; Microbiota; Microbiota analysis |
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Résumé | BACKGROUND: Formation of operational taxonomic units (OTU) is a common approach to data aggregation in microbial ecology studies based on amplification and sequencing of individual gene targets. The de novo assembly of OTU sequences has been recently demonstrated as an alternative to widely used clustering methods, providing robust information from experimental data alone, without any reliance on an external reference database.
RESULTS: Here we introduce mPUMA (microbial Profiling Using Metagenomic Assembly, http://mpuma.sourceforge.net), a software package for identification and analysis of protein-coding barcode sequence data. It was developed originally for Cpn 60 universal target sequences (also known as Gro EL or Hsp 60). Using an unattended process that is independent of external reference sequences, mPUMA forms OTUs by DNA sequence assembly and is capable of tracking OTU abundance. mPUMA processes microbial profiles both in terms of the direct DNA sequence as well as in the translated amino acid sequence for protein coding barcodes. By forming OTUs and calculating abundance through an assembly approach, mPUMA is capable of generating inputs for several popular microbiota analysis tools. Using SFF data from sequencing of a synthetic community of Cpn 60 sequences derived from the human vaginal microbiome, we demonstrate that mPUMA can faithfully reconstruct all expected OTU sequences and produce compositional profiles consistent with actual community structure.
CONCLUSIONS: mPUMA enables analysis of microbial communities while empowering the discovery of novel organisms through OTU assembly. |
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Date de publication | 2013-08-15 |
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Maison d’édition | BioMed Central |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Identificateur | 2049-2618-1-23 |
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Numéro NPARC | 23000568 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 955b5ec2-0dcb-40d9-bd9c-f420ff33cf75 |
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Enregistrement créé | 2016-08-02 |
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Enregistrement modifié | 2020-06-02 |
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