| Téléchargement | - Voir la version finale : Limitations and optimizations of cellular lineages tracking (PDF, 4.8 Mio)
- Voir les données supplémentaires : Limitations and optimizations of cellular lineages tracking (PDF, 1.4 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1371/journal.pcbi.1012880 |
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| Auteur | Rechercher : Leibovich, Nava1Identifiant ORCID : https://orcid.org/0009-0000-9182-3640; Rechercher : Goyal, Sidhartha |
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| Éditeur | Rechercher : Uhlmann, Virginie |
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| Affiliation | - Conseil national de recherches du Canada. Technologies numériques
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| Bailleur de fonds | Rechercher : Medicine by Design (MbD); Rechercher : Natural Sciences and Engineering Research Council of Canada (NSERC) |
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| Format | Texte, Article |
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| Sujet | cloning; RNA sequencing; species diversity; gene pool; vector-borne diseases; experimental design; probability distribution; viral vector techniques |
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| Résumé | Tracking cellular lineages using genetic barcodes provides insights across biology and has become an important tool. However, barcoding strategies remain ad hoc. We show that elevating barcode insertion probability and thus increasing the average number of barcodes within the cells, adds to the number of traceable lineages but may decrease the accuracy of lineages inference due to reading errors. We establish the trade-off between accuracy in tracing lineages and the total number of traceable lineages, and find optimal experimental parameters under limited resources concerning the populations size of tracked cells and barcode pool complexity. |
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| Date de publication | 2025-04-14 |
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| Maison d’édition | Public Library of Science (PLOS) |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 94d06d30-494d-43af-a360-2276803a1476 |
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| Enregistrement créé | 2025-05-08 |
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| Enregistrement modifié | 2025-06-03 |
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