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Gouvernement du Canada / Government of Canada

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Expression systems for therapeutic glycoprotein production

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.copbio.2009.10.008
AuteurRechercher : 1; Rechercher :
Affiliation
  1. Conseil national de recherches Canada. Institut de recherche en biotechnologie du CNRC
FormatTexte, Article
Sujetactivity; apoptosis; baculovirus; bcl-2; biotechnology; bioreactors; blood; caspase 3; cell culture; cell line; cell; chickens; DNA; DNA methylation; endoplasmic reticulum; enzymes; enzymology; epidermal growth factor; galactose; genes; genetic engineering; genome; glycoproteins; glycosylation; heat; HIV-1; IGG; immunoglobulin g; insect cell; lectins; metabolism; microbiology; peptide; Pichia; plant; protein; protein production; rabbit; recombinant proteins; RNA; saccharomyces cerevisiae; secretion; sodium; stress; synthesis; T-lymphocytes; temperature; transfection; tyrosine; yeast
Résumé
There are slightly over 165 recombinant pharmaceuticals currently approved for human use. Another 500 protein candidates are in preclinical and clinical development, about 70% of these being glycosylated proteins. The need for expression systems allowing the efficient manufacturing of high quality glycoproteins is thus becoming imperative. Recent developments with CHO cells, the predominant mammalian expression system, have focused on either increasing cell specific productivity or prolonging the life span of cells in culture that translates to high integrated viable cell densities. These two factors have allowed volumetric productivities in excess of 5 g/L under conditions of controlled nutrient feeding. In addition to glycoengineering strategies, which are offering considerable advantage in producing proteins with enhanced therapeutic properties, several alternative expression systems are being developed for their manufacture, each with their advantages and limitations.
Date de publication
Dans
  • Current Opinion in Biotechnology 20, nº 6 (4 novembre 2009) : 700–707.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 52743
Numéro NPARC14022219
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Identificateur de l’enregistrement94328d3a-c8d6-4175-80a0-ac5e9c57c310
Enregistrement créé2010-06-02
Enregistrement modifié2020-04-16
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