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Critical assessment of current adeno-associated viral vector production and quantification methods

DOITrouver le DOI : https://doi.org/10.1016/j.biotechadv.2007.09.001
AuteurRechercher : 1; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches Canada. Institut de recherche en biotechnologie du CNRC
FormatTexte, Article
SujetAdeno-associated virus; biotechnology; gene therapy; ELISA; therapy; infectious assay; replication assay; transduction assay; review; production; quantification; adeno-associated virus; PCR; methods; therapy
Résumé
Adeno-associated viral vectors have emerged as one of the most studied vectors for gene therapy. Numerous production methods have been described, each with its advantages and disadvantages. A challenge in assessing the current state of the art exists in comparing yields from one production system to the next due to the wide variety of quantification techniques. In this review, AAV vector production methods are summarized and the yields of the different processes are standardized to the number of harvested cells. Titers are further streamlined into five categories: transduction units, enhanced transduction units, infectious particles, DNase-resistant particles and total particles, and the importance of each type of measure is discussed.
Date de publication
Condition d’accès
  • available
  • unlimited
  • public
Dans
  • Biotechnology Advances 26, nº January-February 1 (8 septembre 2007) : 73–88.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRC47808
NRCC 47808
Numéro NPARC8925916
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement92a8aee6-3b96-4023-b4d3-c9662146be65
Enregistrement créé2009-04-23
Enregistrement modifié2020-05-10

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