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Identification of genes differently expressed in Atlantic salmon (Salmo salar) in response to infection by Aermonas salmonicida using cDNA microarray technology

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.dci.2004.08.004
AuteurRechercher : 1; Rechercher : 2; Rechercher : 1; Rechercher : ; Rechercher : 3; Rechercher : 2; Rechercher : 1; Rechercher : 2
Affiliation du nom
  1. Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
  2. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
  3. Conseil national de recherches du Canada. Thérapeutique en santé humaine
FormatTexte, Article
SujetAeromonas salmonicida; atlantic salmon; furunculosis; gene expression; innate immunity; microarray; Salmo salar
Résumé
The response of Atlantic salmon, Salmo salar, to infection by the bacterial pathogen Aeromonas salmonicida (the causative agent of furunculosis), was investigated using a cohabitation model and a custom Atlantic salmon cDNA microarray consisting of over 4000 different amplicons. Pooled samples of each of three immune-relevant tissues (spleen, head kidney and liver) were obtained from fish exposed to infected salmon for 13 days. Reverse transcription-PCR assays were used to verify the differential expression of 12 candidate genes uncovered by microarray analysis. Among the differentially expressed genes were several previously revealed by suppression subtractive hybridization and EST surveys and that are recognized to encode humoral components of the innate immune system. Other genes identified in this study were not previously associated with infection. In addition, a number of genes with no known homologs were uncovered. Determination of their specific roles during infection may lead to a better understanding of innate immunity.
Date de publication
Conditions d’utilisation
  • Crown copyright © 2004 Published by Elsevier Ltd.
Dans
  • Developmental & Comparative Immunology 29, nº 4 (2005) : 333–347.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42446
1411
Numéro NPARC3538500
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Identificateur de l’enregistrement86a1a81b-f791-4c77-9f46-e195d827adaa
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-07
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