| DOI | Trouver le DOI : https://doi.org/10.1105/tpc.114.124743 |
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| Auteur | Rechercher : Borg, M.; Rechercher : Rutley, N.; Rechercher : Kagale, S.1; Rechercher : Hamamura, Y.; Rechercher : Gherghinoiu, M.; Rechercher : Kumar, S.; Rechercher : Sari, U.; Rechercher : Esparza-Franco, M.A.; Rechercher : Sakamoto, W.; Rechercher : Rozwadowski, K.; Rechercher : Higashiyama, T.; Rechercher : Twell, D. |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Résumé | The production of the sperm cells in angiosperms requires coordination of cell division and cell differentiation. In Arabidopsis thaliana, the germline-specific MYB protein DUO1 integrates these processes, but the regulatory hierarchy in which DUO1 functions is unknown. Here, we identify an essential role for two germline-specific DUO1 target genes, DAZ1 and DAZ2, which encode EAR motif-containing C2H2-type zinc finger proteins. We show that DAZ1/DAZ2 are required for germ cell division and for the proper accumulation of mitotic cyclins. Importantly, DAZ1/DAZ2 are sufficient to promote G2- to M-phase transition and germ cell division in the absence of DUO1. DAZ1/DAZ2 are also required for DUO1-dependent cell differentiation and are essential for gamete fusion at fertilization. We demonstrate that the two EAR motifs in DAZ1/DAZ2 mediate their function in the male germline and are required for transcriptional repression and for physical interaction with the corepressor TOPLESS. Our findings uncover an essential module in a regulatory hierarchy that drives mitotic transition in male germ cells and implicates gene repression pathways in sperm cell formation and fertility. |
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| Date de publication | 2014-05 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC-ACRD-56070 |
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| Numéro NPARC | 21272400 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 82d28fab-facc-424a-b1b2-1c986cef320d |
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| Enregistrement créé | 2014-11-25 |
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| Enregistrement modifié | 2020-04-22 |
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