| Téléchargement | - Voir la version finale : Microfluidic integration of magnetically functionalized microwires for flow cytometry protein quantification (PDF, 2.4 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.3390/ma18020215 |
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| Auteur | Rechercher : Clime, Liviu1Identifiant ORCID : https://orcid.org/0000-0002-1786-0803; Rechercher : Pavel, CatalinIdentifiant ORCID : https://orcid.org/0009-0008-6069-0425; Rechercher : Malic, Lidija1Identifiant ORCID : https://orcid.org/0000-0001-8594-9191; Rechercher : Nassif, Christina1; Rechercher : Geissler, Matthias1Identifiant ORCID : https://orcid.org/0000-0002-8838-5528; Rechercher : Lupu, Nicoleta; Rechercher : Óvári, Tibor-AdrianIdentifiant ORCID : https://orcid.org/0000-0003-3074-7749; Rechercher : Poncelet, Lucas1Identifiant ORCID : https://orcid.org/0000-0002-1376-9367; Rechercher : Veilleux, Gaétan1; Rechercher : Moslemi, Elham1; Rechercher : Hernández-Castro, Javier Alejandro1Identifiant ORCID : https://orcid.org/0000-0002-1084-4392; Rechercher : Sinnett, DanielIdentifiant ORCID : https://orcid.org/0000-0003-3625-6676; Rechercher : Che, Diping; Rechercher : Veres, Teodor1Identifiant ORCID : https://orcid.org/0000-0002-9594-8042 |
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| Affiliation | - Conseil national de recherches Canada. Dispositifs médicaux
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| Format | Texte, Article |
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| Sujet | microfluidics; protein quantification; magnetic microwires; magnetic nanoparticles; anocrystalline magnets; flow cytometry |
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| Résumé | A novel approach to protein quantification utilizing a microfluidic platform activated by a magnetic assembly of functionalized magnetic beads around soft magnetic capture centers is presented. Functionalized magnetic beads, known for their high surface area and facile manipulation under external magnetic fields, are injected inside microfluidic channels and immobilized magnetically on the surface of glass-coated soft magnetic microwires placed along the symmetry axis of these channels. A fluorescent (Cy5) immunomagnetic sandwich ELISA is then performed by sequentially flowing the sample and all necessary reagents in the microfluidic channels. Direct protein quantification is performed by magnetically releasing the beads from the microwire and evaluating their fluorescence intensity with the help of a miniature (microfluidic-based) flow cytometer. Measurements of ICAM-1 protein concentration in human blood plasma samples confirm the feasibility of the approach through extensive performance benchmarking. The automation and multiplexing capabilities of the proposed platform further demonstrate its potential for protein quantification in point-of-care settings using microfluidics and miniature flow cytometry instruments. |
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| Date de publication | 2025-01-07 |
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| Maison d’édition | MDPI |
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| Licence | |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 82154c45-c159-4e81-830e-fbfa8bff3cb1 |
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| Enregistrement créé | 2025-06-19 |
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| Enregistrement modifié | 2025-06-19 |
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